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Electron microscopic study of measles virus infection: unusual antibody-triggered redistribution of antigens on giant cells

机译：麻疹病毒感染的电子显微镜研究：巨细胞上异常的抗体触发的抗原重新分布

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Vero cells infected with measles virus fuse to form multinucleated cells which incorporated virus- specific antigens in their membrane. The distribution of these antigens was analyzed after a brief treatment with human anti-measles immunoglobulin G, using autoradiography and immunoperoxidase labeling combined with transmission and scanning electron microscopy. Virus-specific antigens were distributed over the entire surface of giant cells treated at 4°C with human anti-measles immunoglobulin G and labeled Protein A. When cells were shifted to 37°C, labeled antigen-antibody complexes were redistributed in two stages. Patch formation occurred in 5 to 15 min. Later, antigen- antibody complexes became concentrated in a paracentral "ring" rather than typical caps. Patch formation occurred in the presence of metabolic inhibitors, whereas ring formation was inhibited by metabolic inhibitors. These rings contained membrane folds, villi, and viral buds, whereas the rest of the membrane was smooth. In addition, shedding, endocytosis of antigen-antibody complexes, and reexpression of antigens were observed. Antibodies to nonviral membrane antigens induced the same pattern of redistribution. Infected cells treated with anti-measles Fab' fragments maintained a homogeneous distribution of label throughout the experiments. In conclusion, intact immunoglobulins, but not Fab' fragments, were able to induce a dramatic redistribution of viral antigen on the membrane of giant cells infected with measles virus.
机译：被麻疹病毒感染的Vero细胞融合形成多核细胞，该细胞在其膜中掺入了病毒特异性抗原。在用人抗麻疹免疫球蛋白G短暂治疗后，使用放射自显影和免疫过氧化物酶标记结合透射电镜和扫描电子显微镜对这些抗原的分布进行了分析。病毒特异性抗原分布在用人抗麻疹免疫球蛋白G和标记的蛋白A在4°C处理的巨细胞的整个表面上。当细胞转移到37°C时，标记的抗原-抗体复合物分两个阶段重新分布。补丁形成发生在5至15分钟内。后来，抗原-抗体复合物变得集中在中央旁的“环”中，而不是典型的帽中。在存在代谢抑制剂的情况下会发生斑块形成，而环的形成会受到代谢抑制剂的抑制。这些环含有膜褶，绒毛和病毒芽，而膜的其余部分是光滑的。另外，观察到脱落，抗原-抗体复合物的内吞作用和抗原的重新表达。非病毒膜抗原的抗体诱导了相同的再分布模式。在整个实验过程中，用抗麻疹Fab'片段处理的感染细胞保持标记的均匀分布。总之，完整的免疫球蛋白而非Fab'片段能够在麻疹病毒感染的巨细胞膜上引起病毒抗原的急剧重新分布。

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